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KMID : 0364020120450060368
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Korean Journal of Thoracic and Cardiovascular Surgery
2012 Volume.45 No. 6 p.368 ~ p.379
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Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and α-Galactosidase: A Comparative Study
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Min Byoung-Ju
Kim Yong-Jin
Choi Jae-Woong
Choi Sun-Young
Kim Soo-Hwan
Lim Hong-Gook
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Abstract
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Background: Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. ?- galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with ?-galactosidase. Materials and Methods: Bovine pericardial tissues were allocated into three groups: fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with ?-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test. Results: Decellularization and elimination of ?-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with ?-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001). Conclusion: Favorable decellularization and elimination of ?-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of ?-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate bioprosthesis with decellularization and elimination of ?-gal including favorable mechanical properties too.
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KEYWORD
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Bioprosthesis, Decellularization, Glutaraldehyde, α-Gal
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